Detection of influenza A virus homo‐ and heterosubtype‐specific memory B‐cells using a novel protein microarray‐based analysis tool
Identifieur interne : 000952 ( Main/Exploration ); précédent : 000951; suivant : 000953Detection of influenza A virus homo‐ and heterosubtype‐specific memory B‐cells using a novel protein microarray‐based analysis tool
Auteurs : Dominique C. Baas [Pays-Bas] ; Marion P. Koopmans [Pays-Bas] ; Erwin De Bruin [Pays-Bas] ; Hinke I Ten Hulscher [Pays-Bas] ; Anne M. Buisman [Pays-Bas] ; Lotte H. Hendrikx [Pays-Bas] ; Janko Van Beek [Pays-Bas] ; Gert-Jan Godeke [Pays-Bas] ; Johan Reimerink [Pays-Bas] ; Robert S. Van Binnendijk [Pays-Bas]Source :
- Journal of Medical Virology [ 0146-6615 ] ; 2013-05.
English descriptors
- Teeft :
- Adult persons, Analysis method, Antibodies binding, Antibody, Antibody analysis, Antibody binding, Antibody responses, Antigen microarray, Antigenic site, Array experiment, Blood sample, Blood samples, Buisman, Bulk culture, Corresponding supernatants, Corti, Crotty, Culture supernatants, Different time points, Elispot analysis, Epitope, Grant number, Grant sponsor, Immune, Immune protection, Immunol, Individual cultures, Infection, Infection attack rates, Infectious diseases, Isogen life sciences, Koopmans, Laboratory cases, Lanzavecchia, Larger degree, Memory reactivity, Memory responses, Memory signals, Microarray, Miltenyi biotec, Multiplex analysis tool, National institute, Netherlands, Neutralizing, Neutralizing antibodies, Pandemic, Pandemic cases, Pandemic strains, Pandemic virus, Pandemic wave, Peripheral blood, Perkin elmer, Pinna, Plasma cells, Pool pool, Proc natl acad, Protein array, Protein microarray, Public health, Recombinant monomeric, Serum responses, Serum samples, Single epitope, Single memory, Single memory precursor, Standard deviation, Subtypes, Supernatant, Unbound cells, Uorescence signal, Uorescence signals, Vaccine, Virol, Virus, Virus antibodies, Virus antibody, Virus infection, Virus neutralization, Virus strain, Virus strains, Virus subtypes, Wiley periodicals.
Abstract
The emergence of the A(H1N1) 2009 pandemic influenza virus was initially seen as a major world‐wide health concern since a low degree of immunity to this virus strain was anticipated. However, age‐specific infection attack rates and age‐specific differences in seroresponse indicate that pre‐existing immunity may have played a significant role in protection especially in older age groups. This study describes the use of a protein microarray as a multiplex analysis tool for detection of influenza virus H1 strain‐specific memory B‐cells before and after infection with A(H1N1)pdm09. The discrimination was based on detection of specific antibodies in culture supernatants from polyclonally stimulated B‐cells against recombinant influenza virus HA1 proteins representing influenza virus subtypes H1 through H9. The protein microarray proved sensitive and specific for antibody detection in culture supernatants of B‐cells, and with the potential to deduce a person's history of infection with particular influenza virus variants, including A(H1N1)pdm09. Blood samples obtained from different age groups prior to the pandemic in 2009 partly showed the presence of B‐cells producing antibodies binding to the closely related A(H1N1) 1918 pandemic influenza virus, and of which the magnitude increased with age. These cross‐reactive antibodies were produced by single memory B‐cells present in these donors, and either bind to epitopes on HA1 which are shared within different H1 strains (homosubtypic response) or shared between different subtypes (heterosubtypic response). J. Med. Virol. 85:899–909, 2013. © 2013 Wiley Periodicals, Inc.
Url:
DOI: 10.1002/jmv.23535
Affiliations:
Links toward previous steps (curation, corpus...)
- to stream Istex, to step Corpus: 001442
- to stream Istex, to step Curation: 001442
- to stream Istex, to step Checkpoint: 000062
- to stream Main, to step Merge: 000953
- to stream Main, to step Curation: 000952
Le document en format XML
<record><TEI wicri:istexFullTextTei="biblStruct"><teiHeader><fileDesc><titleStmt><title xml:lang="en">Detection of influenza A virus homo‐ and heterosubtype‐specific memory B‐cells using a novel protein microarray‐based analysis tool</title><author><name sortKey="Baas, Dominique C" sort="Baas, Dominique C" uniqKey="Baas D" first="Dominique C." last="Baas">Dominique C. Baas</name></author><author><name sortKey="Koopmans, Marion P" sort="Koopmans, Marion P" uniqKey="Koopmans M" first="Marion P." last="Koopmans">Marion P. Koopmans</name></author><author><name sortKey="De Bruin, Erwin" sort="De Bruin, Erwin" uniqKey="De Bruin E" first="Erwin" last="De Bruin">Erwin De Bruin</name></author><author><name sortKey="Hulscher, Hinke I Ten" sort="Hulscher, Hinke I Ten" uniqKey="Hulscher H" first="Hinke I Ten" last="Hulscher">Hinke I Ten Hulscher</name></author><author><name sortKey="Buisman, Anne M" sort="Buisman, Anne M" uniqKey="Buisman A" first="Anne M." last="Buisman">Anne M. Buisman</name></author><author><name sortKey="Hendrikx, Lotte H" sort="Hendrikx, Lotte H" uniqKey="Hendrikx L" first="Lotte H." last="Hendrikx">Lotte H. Hendrikx</name></author><author><name sortKey="Van Beek, Janko" sort="Van Beek, Janko" uniqKey="Van Beek J" first="Janko" last="Van Beek">Janko Van Beek</name></author><author><name sortKey="Godeke, Gert An" sort="Godeke, Gert An" uniqKey="Godeke G" first="Gert-Jan" last="Godeke">Gert-Jan Godeke</name></author><author><name sortKey="Reimerink, Johan" sort="Reimerink, Johan" uniqKey="Reimerink J" first="Johan" last="Reimerink">Johan Reimerink</name></author><author><name sortKey="Van Binnendijk, Robert S" sort="Van Binnendijk, Robert S" uniqKey="Van Binnendijk R" first="Robert S." last="Van Binnendijk">Robert S. Van Binnendijk</name></author></titleStmt><publicationStmt><idno type="wicri:source">ISTEX</idno><idno type="RBID">ISTEX:684AEA0E5866579486353735C9586FB758058643</idno><date when="2013" year="2013">2013</date><idno type="doi">10.1002/jmv.23535</idno><idno type="url">https://api.istex.fr/ark:/67375/WNG-PFXZ0HMS-B/fulltext.pdf</idno><idno type="wicri:Area/Istex/Corpus">001442</idno><idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Corpus" wicri:corpus="ISTEX">001442</idno><idno type="wicri:Area/Istex/Curation">001442</idno><idno type="wicri:Area/Istex/Checkpoint">000062</idno><idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Checkpoint">000062</idno><idno type="wicri:doubleKey">0146-6615:2013:Baas D:detection:of:influenza</idno><idno type="wicri:Area/Main/Merge">000953</idno><idno type="wicri:Area/Main/Curation">000952</idno><idno type="wicri:Area/Main/Exploration">000952</idno></publicationStmt><sourceDesc><biblStruct><analytic><title level="a" type="main">Detection of influenza A virus homo‐ and heterosubtype‐specific memory B‐cells using a novel protein microarray‐based analysis tool</title><author><name sortKey="Baas, Dominique C" sort="Baas, Dominique C" uniqKey="Baas D" first="Dominique C." last="Baas">Dominique C. Baas</name><affiliation wicri:level="1"><country xml:lang="fr" wicri:curation="lc">Pays-Bas</country><wicri:regionArea>Laboratory for Infectious Diseases and Screening, Center for Infectious Disease Control, Center for Infectious Disease Research, Diagnostics and Screening, National Institute for Public Health and the Environment, Bilthoven</wicri:regionArea><wicri:noRegion>Bilthoven</wicri:noRegion></affiliation></author><author><name sortKey="Koopmans, Marion P" sort="Koopmans, Marion P" uniqKey="Koopmans M" first="Marion P." last="Koopmans">Marion P. Koopmans</name><affiliation wicri:level="1"><country xml:lang="fr" wicri:curation="lc">Pays-Bas</country><wicri:regionArea>Laboratory for Infectious Diseases and Screening, Center for Infectious Disease Control, Center for Infectious Disease Research, Diagnostics and Screening, National Institute for Public Health and the Environment, Bilthoven</wicri:regionArea><wicri:noRegion>Bilthoven</wicri:noRegion></affiliation></author><author><name sortKey="De Bruin, Erwin" sort="De Bruin, Erwin" uniqKey="De Bruin E" first="Erwin" last="De Bruin">Erwin De Bruin</name><affiliation wicri:level="1"><country xml:lang="fr" wicri:curation="lc">Pays-Bas</country><wicri:regionArea>Laboratory for Infectious Diseases and Screening, Center for Infectious Disease Control, Center for Infectious Disease Research, Diagnostics and Screening, National Institute for Public Health and the Environment, Bilthoven</wicri:regionArea><wicri:noRegion>Bilthoven</wicri:noRegion></affiliation></author><author><name sortKey="Hulscher, Hinke I Ten" sort="Hulscher, Hinke I Ten" uniqKey="Hulscher H" first="Hinke I Ten" last="Hulscher">Hinke I Ten Hulscher</name><affiliation wicri:level="1"><country xml:lang="fr" wicri:curation="lc">Pays-Bas</country><wicri:regionArea>Laboratory for Infectious Diseases and Screening, Center for Infectious Disease Control, Center for Infectious Disease Research, Diagnostics and Screening, National Institute for Public Health and the Environment, Bilthoven</wicri:regionArea><wicri:noRegion>Bilthoven</wicri:noRegion></affiliation></author><author><name sortKey="Buisman, Anne M" sort="Buisman, Anne M" uniqKey="Buisman A" first="Anne M." last="Buisman">Anne M. Buisman</name><affiliation wicri:level="1"><country xml:lang="fr" wicri:curation="lc">Pays-Bas</country><wicri:regionArea>Laboratory for Infectious Diseases and Screening, Center for Infectious Disease Control, Center for Infectious Disease Research, Diagnostics and Screening, National Institute for Public Health and the Environment, Bilthoven</wicri:regionArea><wicri:noRegion>Bilthoven</wicri:noRegion></affiliation></author><author><name sortKey="Hendrikx, Lotte H" sort="Hendrikx, Lotte H" uniqKey="Hendrikx L" first="Lotte H." last="Hendrikx">Lotte H. Hendrikx</name><affiliation wicri:level="1"><country xml:lang="fr" wicri:curation="lc">Pays-Bas</country><wicri:regionArea>Laboratory for Infectious Diseases and Screening, Center for Infectious Disease Control, Center for Infectious Disease Research, Diagnostics and Screening, National Institute for Public Health and the Environment, Bilthoven</wicri:regionArea><wicri:noRegion>Bilthoven</wicri:noRegion></affiliation></author><author><name sortKey="Van Beek, Janko" sort="Van Beek, Janko" uniqKey="Van Beek J" first="Janko" last="Van Beek">Janko Van Beek</name><affiliation wicri:level="1"><country xml:lang="fr" wicri:curation="lc">Pays-Bas</country><wicri:regionArea>Laboratory for Infectious Diseases and Screening, Center for Infectious Disease Control, Center for Infectious Disease Research, Diagnostics and Screening, National Institute for Public Health and the Environment, Bilthoven</wicri:regionArea><wicri:noRegion>Bilthoven</wicri:noRegion></affiliation></author><author><name sortKey="Godeke, Gert An" sort="Godeke, Gert An" uniqKey="Godeke G" first="Gert-Jan" last="Godeke">Gert-Jan Godeke</name><affiliation wicri:level="1"><country xml:lang="fr" wicri:curation="lc">Pays-Bas</country><wicri:regionArea>Laboratory for Infectious Diseases and Screening, Center for Infectious Disease Control, Center for Infectious Disease Research, Diagnostics and Screening, National Institute for Public Health and the Environment, Bilthoven</wicri:regionArea><wicri:noRegion>Bilthoven</wicri:noRegion></affiliation></author><author><name sortKey="Reimerink, Johan" sort="Reimerink, Johan" uniqKey="Reimerink J" first="Johan" last="Reimerink">Johan Reimerink</name><affiliation wicri:level="1"><country xml:lang="fr" wicri:curation="lc">Pays-Bas</country><wicri:regionArea>Laboratory for Infectious Diseases and Screening, Center for Infectious Disease Control, Center for Infectious Disease Research, Diagnostics and Screening, National Institute for Public Health and the Environment, Bilthoven</wicri:regionArea><wicri:noRegion>Bilthoven</wicri:noRegion></affiliation></author><author><name sortKey="Van Binnendijk, Robert S" sort="Van Binnendijk, Robert S" uniqKey="Van Binnendijk R" first="Robert S." last="Van Binnendijk">Robert S. Van Binnendijk</name><affiliation wicri:level="1"><country xml:lang="fr" wicri:curation="lc">Pays-Bas</country><wicri:regionArea>Laboratory for Infectious Diseases and Screening, Center for Infectious Disease Control, Center for Infectious Disease Research, Diagnostics and Screening, National Institute for Public Health and the Environment, Bilthoven</wicri:regionArea><wicri:noRegion>Bilthoven</wicri:noRegion></affiliation><affiliation wicri:level="1"><country wicri:rule="url">Pays-Bas</country></affiliation><affiliation wicri:level="1"><country xml:lang="fr" wicri:curation="lc">Pays-Bas</country><wicri:regionArea>Correspondence address: Laboratory for Infectious Diseases and Screening, National Institute for Public Health and the Environment (RIVM), P.O. Box 1, 3720 BA Bilthoven</wicri:regionArea><wicri:noRegion>3720 BA Bilthoven</wicri:noRegion></affiliation></author></analytic><monogr></monogr><series><title level="j" type="main">Journal of Medical Virology</title><title level="j" type="alt">JOURNAL OF MEDICAL VIROLOGY</title><idno type="ISSN">0146-6615</idno><idno type="eISSN">1096-9071</idno><imprint><biblScope unit="vol">85</biblScope><biblScope unit="issue">5</biblScope><biblScope unit="page" from="899">899</biblScope><biblScope unit="page" to="909">909</biblScope><biblScope unit="page-count">11</biblScope><publisher>Wiley Subscription Services, Inc., A Wiley Company</publisher><pubPlace>Hoboken</pubPlace><date type="published" when="2013-05">2013-05</date></imprint><idno type="ISSN">0146-6615</idno></series></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0146-6615</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="Teeft" xml:lang="en"><term>Adult persons</term><term>Analysis method</term><term>Antibodies binding</term><term>Antibody</term><term>Antibody analysis</term><term>Antibody binding</term><term>Antibody responses</term><term>Antigen microarray</term><term>Antigenic site</term><term>Array experiment</term><term>Blood sample</term><term>Blood samples</term><term>Buisman</term><term>Bulk culture</term><term>Corresponding supernatants</term><term>Corti</term><term>Crotty</term><term>Culture supernatants</term><term>Different time points</term><term>Elispot analysis</term><term>Epitope</term><term>Grant number</term><term>Grant sponsor</term><term>Immune</term><term>Immune protection</term><term>Immunol</term><term>Individual cultures</term><term>Infection</term><term>Infection attack rates</term><term>Infectious diseases</term><term>Isogen life sciences</term><term>Koopmans</term><term>Laboratory cases</term><term>Lanzavecchia</term><term>Larger degree</term><term>Memory reactivity</term><term>Memory responses</term><term>Memory signals</term><term>Microarray</term><term>Miltenyi biotec</term><term>Multiplex analysis tool</term><term>National institute</term><term>Netherlands</term><term>Neutralizing</term><term>Neutralizing antibodies</term><term>Pandemic</term><term>Pandemic cases</term><term>Pandemic strains</term><term>Pandemic virus</term><term>Pandemic wave</term><term>Peripheral blood</term><term>Perkin elmer</term><term>Pinna</term><term>Plasma cells</term><term>Pool pool</term><term>Proc natl acad</term><term>Protein array</term><term>Protein microarray</term><term>Public health</term><term>Recombinant monomeric</term><term>Serum responses</term><term>Serum samples</term><term>Single epitope</term><term>Single memory</term><term>Single memory precursor</term><term>Standard deviation</term><term>Subtypes</term><term>Supernatant</term><term>Unbound cells</term><term>Uorescence signal</term><term>Uorescence signals</term><term>Vaccine</term><term>Virol</term><term>Virus</term><term>Virus antibodies</term><term>Virus antibody</term><term>Virus infection</term><term>Virus neutralization</term><term>Virus strain</term><term>Virus strains</term><term>Virus subtypes</term><term>Wiley periodicals</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">The emergence of the A(H1N1) 2009 pandemic influenza virus was initially seen as a major world‐wide health concern since a low degree of immunity to this virus strain was anticipated. However, age‐specific infection attack rates and age‐specific differences in seroresponse indicate that pre‐existing immunity may have played a significant role in protection especially in older age groups. This study describes the use of a protein microarray as a multiplex analysis tool for detection of influenza virus H1 strain‐specific memory B‐cells before and after infection with A(H1N1)pdm09. The discrimination was based on detection of specific antibodies in culture supernatants from polyclonally stimulated B‐cells against recombinant influenza virus HA1 proteins representing influenza virus subtypes H1 through H9. The protein microarray proved sensitive and specific for antibody detection in culture supernatants of B‐cells, and with the potential to deduce a person's history of infection with particular influenza virus variants, including A(H1N1)pdm09. Blood samples obtained from different age groups prior to the pandemic in 2009 partly showed the presence of B‐cells producing antibodies binding to the closely related A(H1N1) 1918 pandemic influenza virus, and of which the magnitude increased with age. These cross‐reactive antibodies were produced by single memory B‐cells present in these donors, and either bind to epitopes on HA1 which are shared within different H1 strains (homosubtypic response) or shared between different subtypes (heterosubtypic response). J. Med. Virol. 85:899–909, 2013. © 2013 Wiley Periodicals, Inc.</div></front></TEI><affiliations><list><country><li>Pays-Bas</li></country></list><tree><country name="Pays-Bas"><noRegion><name sortKey="Baas, Dominique C" sort="Baas, Dominique C" uniqKey="Baas D" first="Dominique C." last="Baas">Dominique C. Baas</name></noRegion><name sortKey="Buisman, Anne M" sort="Buisman, Anne M" uniqKey="Buisman A" first="Anne M." last="Buisman">Anne M. Buisman</name><name sortKey="De Bruin, Erwin" sort="De Bruin, Erwin" uniqKey="De Bruin E" first="Erwin" last="De Bruin">Erwin De Bruin</name><name sortKey="Godeke, Gert An" sort="Godeke, Gert An" uniqKey="Godeke G" first="Gert-Jan" last="Godeke">Gert-Jan Godeke</name><name sortKey="Hendrikx, Lotte H" sort="Hendrikx, Lotte H" uniqKey="Hendrikx L" first="Lotte H." last="Hendrikx">Lotte H. Hendrikx</name><name sortKey="Hulscher, Hinke I Ten" sort="Hulscher, Hinke I Ten" uniqKey="Hulscher H" first="Hinke I Ten" last="Hulscher">Hinke I Ten Hulscher</name><name sortKey="Koopmans, Marion P" sort="Koopmans, Marion P" uniqKey="Koopmans M" first="Marion P." last="Koopmans">Marion P. Koopmans</name><name sortKey="Reimerink, Johan" sort="Reimerink, Johan" uniqKey="Reimerink J" first="Johan" last="Reimerink">Johan Reimerink</name><name sortKey="Van Beek, Janko" sort="Van Beek, Janko" uniqKey="Van Beek J" first="Janko" last="Van Beek">Janko Van Beek</name><name sortKey="Van Binnendijk, Robert S" sort="Van Binnendijk, Robert S" uniqKey="Van Binnendijk R" first="Robert S." last="Van Binnendijk">Robert S. Van Binnendijk</name><name sortKey="Van Binnendijk, Robert S" sort="Van Binnendijk, Robert S" uniqKey="Van Binnendijk R" first="Robert S." last="Van Binnendijk">Robert S. Van Binnendijk</name><name sortKey="Van Binnendijk, Robert S" sort="Van Binnendijk, Robert S" uniqKey="Van Binnendijk R" first="Robert S." last="Van Binnendijk">Robert S. Van Binnendijk</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Sante/explor/H2N2V1/Data/Main/Exploration
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000952 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 000952 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Sante
|area= H2N2V1
|flux= Main
|étape= Exploration
|type= RBID
|clé= ISTEX:684AEA0E5866579486353735C9586FB758058643
|texte= Detection of influenza A virus homo‐ and heterosubtype‐specific memory B‐cells using a novel protein microarray‐based analysis tool
}}
| This area was generated with Dilib version V0.6.33. |  |